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Alteration of heat shock protein 20 expression in preeclamptic patients and its effect in vascular and

Fanfan Li, Mengzhou He, Meitao Yang, Yao Fan, Yun Chen, Xi Xia, Yin Xie, Dongrui Deng

《医学前沿（英文）》 2018年 第12卷 第5期   页码 542-549 doi: 10.1007/s11684-017-0576-x

摘要：

Preeclampsia (PE) is a pregnancy-specific, multi-system disorder and the leading cause of maternal and perinatal morbidity and mortality in obstetrics worldwide. Excessive vasoconstriction and dysregulated coagulation function are closely associated with PE. Heat shock protein 20 (HSP20) is ubiquitously expressed under normal physiological conditions and has important roles in vascular dilatation and suppression of platelet aggregation. However, the role of HSP20 in the pathogenesis of PE remains unclear. In this study, we collected chorionic plate resistance arteries (CPAs) and serum from 118 healthy pregnant women and 80 women with PE and detected the levels of HSP20 and its phosphorylated form. Both HSP20 and phosphorylated HSP20 were downregulated in CPAs from women with PE. Comparison of the vasodilative ability of CPAs from the two groups showed impaired relaxation responses to acetyl choline in preeclamptic vessels. In addition to the reduced HSP20 in serum from women with PE, the platelet distribution width and mean platelet volume were also decreased, and the activated partial thromboplastin time and thromboplastin time were elevated. With regard to the vital roles of HSP20 in mediating vasorelaxation and coagulation function, the decreased HSP20 might contribute to the pathogenesis of PE.

关键词： preeclampsia     heat shock protein 20     vascular relaxation     coagulation-fibrinolytic system    

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Expression of heat shock protein gp96 in osteosarcoma and its clinical significance

GUO Weichun, CHEN Anmin

《医学前沿（英文）》 2008年 第2卷 第2期   页码 200-203 doi: 10.1007/s11684-008-0038-6

摘要： This study aims to detect the expression of heat shock protein gp96 (HSPgp96) in osteosarcoma and explore its role in anti-tumor immunity. Expression of HSPgp96 in 45 cases of osteosarcoma, including 24 cases of the osteoblast type, 21 cases of chondroblast type and 5 cases of normal bone tissue were detected by immunohistochemistry (IHC). The expression rate of HSPgp96 was 100% (45/45) in osteosarcoma, compared with 20% (1/5) in normal bone tissue. The average positive cell rates were 97.23% and 0.48% in osteosarcoma and normal bone tissue, respectively, and the difference between them was significant ( < 0.01). The positive cell rates in osteoblast type and chondroblast type were 97.78% and 96.56%, respectively, and the difference had no statistical significance ( > 0.05). The expression degree of HSPgp96 in osteosarcoma had a clear correlation with the pathologic degree ( < 0.05), while it had no relationship with the clinical stage ( > 0.05). The expression of HSPgp96 is high in osteosarcoma and it may be a significant index for judgment of malignancy degree and an important target site of anti-tumor immunity.
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Development of a dual temperature control system for isoprene biosynthesis in

《化学科学与工程前沿（英文）》 2022年 第16卷 第7期   页码 1079-1089 doi: 10.1007/s11705-021-2088-0

摘要： Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis of secondary metabolites. To address the growth-production conflict in yeast strains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesis was explored. A dual temperature regulation system was developed through engineering and expression regulation of the transcriptional activator Gal4p. A cold-sensitive mutant, Gal4ep19, was created by directed evolution of Gal4p based on an internally developed growth-based high-throughput screening method and expressed under the heat-shock promoter PSSA4 to control the expression of PGAL-driven pathway genes in the mitochondria. Compared to the control strain with constitutively expressed wild-type Gal4p, the dual temperature regulation strategy led to 34.5% and 72% improvements in cell growth and isoprene production, respectively. This study reports the creation of the first cold-sensitive variants of Gal4p by directed evolution and provides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesis of other high-value natural products.

关键词： transcriptional activator     directed evolution     dynamic control     heat-shock     isoprene    

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Beneficial effect of arginine vasopressin on hemorrhagic shock through improving the vascular reactivity

LI Tao, YANG Guangming, XU Jing, LIU Jiancang, LIU Liangming

《医学前沿（英文）》 2008年 第2卷 第3期   页码 248-254 doi: 10.1007/s11684-008-0047-5

摘要： The vascular reactivity and calcium sensitivity were decreased following hemorrhagic shock. Arginine vasopressin (AVP) was beneficial to endotoxic, infectious/septic and hemorrhagic shock. Our previous studies found that Rho kinase played an important role in the occurrence of calcium desensitization following shock. It was reported that AVP was with stimulation effect of Rho kinase. So we hypothesized that AVP might have beneficial effect on shock via activation of Rho kinase to regulate the calcium sensitivity and vascular reactivity. Hemorrhagic shock (40 mmHg for 2 h) Wistar rats were adopted to observe the effects of small dose of AVP on hemodynamics, 24-h survival rate, the pressor effect of norepinephrine (NE) and the contractility of superior mesenteric artery (SMA). Isolated SMAs from hemorrhagic shock rats were adopted to observe the effects of AVP on vascular reactivity and calcium sensitivity and its relationship to Rho kinase with an isolated organ perfusion system. The results show that AVP at the concentration of 0.1 U/kg and 0.4 U/kg significantly improved the hemodynamic parameters and the 24-h survival rate of hemorrhagic shock rats. Meanwhile, these dosages of AVP significantly increased the pressor effect of NE and the contractile response of SMA to NE. Y-27632 (3 ?g/kg), a Rho kinase specific inhibitor, abolished the beneficial effects of AVP. , the calcium sensitivity and vascular reactivity of SMA to calcium and NE were significantly decreased following hemorrhagic shock. AVP at the concentration of 0.5 nmol/L and 5 nmol/L significantly increased the calcium sensitivity and vascular reactivity. These effects of AVP were abolished by Y-27632 (10 ?mol/L). Taken together, the results suggest that AVP at 0.1 U/kg and 0.4 U/kg is beneficial to hemorrhagic shock by improving the vascular reactivity, which involves activation of Rho kinase.
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Nonlinear dynamic analysis of functionally graded carbon nanotube-reinforced composite plates using MISQ20

《结构与土木工程前沿（英文）》   页码 1072-1085 doi: 10.1007/s11709-023-0951-4

摘要： The main objective of this study is to further extend the mixed integration smoothed quadrilateral element with 20 unknowns of displacement (MISQ20) to investigate the nonlinear dynamic responses of functionally graded carbon nanotube-reinforced composite (FG-CNTRC) plates with four types of carbon nanotube distributions. The smooth finite element method is used to enhance the accuracy of the Q4 element and avoid shear locking without using any shear correction factors. This method yields accurate results even if the element exhibits a concave quadrilateral shape and reduces the error when the element meshing is rough. Additionally, the element stiffness matrix is established by integrating the boundary of the smoothing domains. The motion equation of the FG-CNTRC plates is solved by adapting the Newmark method combined with the Newton–Raphson algorithm. Subsequently, the calculation program is coded in the MATLAB software and verified by comparing it with other published solutions. Finally, the effects of the input parameters on the nonlinear vibration of the plates are investigated.

关键词： carbon nanotube     MISQ20     FG-CNTRC plate     nonlinear vibration     nonlinear dynamic analysis     SFEM    

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Rolling-element bearings in China: From ancient times to the 20th century

Lie SUN,Ang LI

《机械工程前沿（英文）》 2016年 第11卷 第1期   页码 33-43 doi: 10.1007/s11465-016-0373-2

摘要：

The development of rolling-element bearings in China has spanned a long period. Based on several typical and important cases, the present article reconstructs the history of rolling-element bearings in China by dividing it into four stages according to the various characteristics of the bearings. The first stage represents the origin of rolling bearings in China, which remains controversial because of several suspected races and cages that were likely the components of bearings more than a millennium ago. At the second stage, a type of simple roller bearing was used for astronomical instruments not later than the 13th century based on clear philological and physical evidence. A similar bearing was also applied to an abridged armillary in the 17th century. Another type of spherical thrust bearings with rolling elements, which is a key component of a traditional Chinese windmill, could support a rotating shaft that moves rotationally and at an angle. At the third stage, the Chinese began studying and using the so-called European-style bearing since the 17th century. Moreover, over the last 100 years, the modern rolling bearing industry was gradually established in China, particularly because of the technology transfer from the Soviet Union in the 1950s. At the fourth stage, the Chinese government initiated the relatively rapid development of bearing technology. The government launched the “bearing movement” from the 1950s to the 1960s to establish the modern bearing industry and to promote rolling bearings as replacement for traditional sliding bearings. Furthermore, a number of large professional factories and institutions in China have continually introduced advanced technology and equipment. At present, these companies and institutions play a significant role in the international bearing industry.

关键词： bearing movement     rolling-element bearing     China     early rolling bearings     technology transfer    

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Centrifuge experiment and numerical analysis of an air-backed plate subjected to underwater shock loading

Zhijie HUANG, Xiaodan REN, Zuyu CHEN, Daosheng LING

《结构与土木工程前沿（英文）》 2019年 第13卷 第6期   页码 1350-1362 doi: 10.1007/s11709-019-0559-x

摘要： In this study, systematic centrifuge experiments and numerical studies are conducted to investigate the effect of shock loads due to an underwater explosion on the dynamic responses of an air-backed steel plate. Numerical simulations with three different models of pressure time history generated by underwater explosion were carried out. The first model of pressure time history was measured in test. The second model to predict the time history of shock wave pressure from an underwater explosion was created by Cole in 1948. Coefficients of Cole’s formulas are determined experimentally. The third model was developed by Zamyshlyaev and Yakovlev in 1973. All of them are implemented into the numerical model to calculate the shock responses of the plate. Simulated peak strains obtained from the three models are compared with the experimental results, yielding average relative differences of 21.39%, 45.73%, and 13.92%, respectively. The Russell error technique is used to quantitatively analyze the correlation between the numerical and experimental results. Quantitative analysis shows that the simulated strains for most measurement points on the steel plate are acceptable. By changing the scaled distances, different shock impulses were obtained and exerted on the steel plate. Systematic numerical studies are performed to investigate the effect of the accumulated shock impulse on the peak strains. The numerical and experimental results suggest that the peak strains are strongly dependent on the accumulated shock impulse.

关键词： underwater explosion     centrifuge experiment     shock load     dynamic response     accumulated shock impulse    

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“20世纪我国重大工程技术成就” 评选结果在京揭晓

null

《中国工程科学》 2002年 第4卷 第1期   页码 94-94

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ORGANIC CARBON STOCKS IN A SILTY TEXTURED SOIL FOLLOWING REINTEGRATION OF A 20 YEARS OLD × SITE INTO

《农业科学与工程前沿（英文）》 2023年 第10卷 第2期   页码 183-197 doi: 10.15302/J-FASE-2023485

摘要：

● 0.98 Mg·ha−1·yr−1 Corg accumulation under miscanthus over 26 years.

关键词： biobased energy crops     C balance     humus accumulation    

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Green building research from design to operation in the past 20 years: A perspective

Borong LIN, Yi WANG, Jiaping LIU, Juan YU

《结构与土木工程前沿（英文）》 2020年 第14卷 第5期   页码 1049-1055 doi: 10.1007/s11709-020-0668-6

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大秦线开行20 kt级重载组合列车系统集成与创新

耿志修

《中国工程科学》 2008年 第10卷 第3期   页码 31-43

摘要： 技术的结合，800 MHz数据电台与Locotrol技术的结合，单套Locotrol系统与SS4机车的结合，以及2台和谐型大功率机车加可控列尾的创新方式，开发了重载配套装备，通过集成创新，使大秦线开行20

关键词： 中国铁路     大秦线     20 kt级重载组合列车     集成创新    

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新型20 m桥梁检测车结构的有限元力学分析

沈意平,何福泉,王送来,张田

《中国工程科学》 2010年 第12卷 第9期   页码 107-112

摘要：

针对设计的新型20 m桥梁检测车，利用Pro/E软件建立了4种工况下整车结构的实体模型，运用有限元软件ANSYS对建立的模型进行了静力和模态分析

关键词： 桥梁检测车     有限元     静力     模态     强度    

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Influence evaluation of loading conditions during pressurized thermal shock transients based on thermal-hydraulics

Jinya KATSUYAMA, Shumpei UNO, Tadashi WATANABE, Yinsheng LI

《机械工程前沿（英文）》 2018年 第13卷 第4期   页码 563-570 doi: 10.1007/s11465-018-0487-9

摘要：

The thermal hydraulic (TH) behavior of coo-lant water is a key factor in the structural integrity assessments on reactor pressure vessels (RPVs) of pressurized water reactors (PWRs) under pressurized thermal shock (PTS) events, because the TH behavior may affect the loading conditions in the assessment. From the viewpoint of TH behavior, configuration of plant equipment and their dimensions, and operator action time considerably influence various parameters, such as the temperature and flow rate of coolant water and inner pressure. In this study, to investigate the influence of the operator action time on TH behavior during a PTS event, we developed an analysis model for a typical Japanese PWR plant, including the RPV and the main components of both primary and secondary systems, and performed TH analyses by using a system analysis code called RELAP5. We applied two different operator action times based on the Japanese and the United States (US) rules: Operators may act after 10 min (Japanese rules) and 30 min (the US rules) after the occurrence of PTS events. Based on the results of TH analysis with different operator action times, we also performed structural analyses for evaluating thermal-stress distributions in the RPV during PTS events as loading conditions in the structural integrity assessment. From the analysis results, it was clarified that differences in operator action times significantly affect TH behavior and loading conditions, as the Japanese rule may lead to lower stresses than that under the US rule because an earlier operator action caused lower pressure in the RPV.

关键词： structural integrity     reactor pressure vessel     pressurized thermal shock     thermal hydraulic analysis     pressurized water reactor     weld residual stress    

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Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction

Hong LI,Ziding ZHANG

《农业科学与工程前沿（英文）》 2016年 第3卷 第2期   页码 102-112 doi: 10.15302/J-FASE-2016100

摘要： Plants are frequently affected by pathogen infections. To effectively defend against such infections, two major modes of innate immunity have evolved in plants; pathogen-associated molecular pattern-triggered immunity and effector-triggered immunity. Although the molecular components as well as the corresponding pathways involved in these two processes have been identified, many aspects of the molecular mechanisms of the plant immune system remain elusive. Recently, the rapid development of omics techniques (e.g., genomics, proteomics and transcriptomics) has provided a great opportunity to explore plant–pathogen interactions from a systems perspective and studies on protein–protein interactions (PPIs) between plants and pathogens have been carried out and characterized at the network level. In this review, we introduce experimental and computational identification methods of PPIs, popular PPI network analysis approaches, and existing bioinformatics resources/tools related to PPIs. Then, we focus on reviewing the progress in genome-wide PPI networks related to plant–pathogen interactions, including pathogen-centric PPI networks, plant-centric PPI networks and interspecies PPI networks between plants and pathogens. We anticipate genome-wide PPI network analysis will provide a clearer understanding of plant–pathogen interactions and will offer some new opportunities for crop protection and improvement.

关键词： plant–pathogen interactions     systems biology     omics     plant immunity     protein–protein interaction     network    

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Preclinical characterization and comparison between CD3/CD19 bispecific and novel CD3/CD19/CD20 trispecific

《医学前沿（英文）》 2022年 第16卷 第1期   页码 139-149 doi: 10.1007/s11684-021-0835-8

摘要： The CD19-targeting bispecific T-cell engager blinatumomab has shown remarkable efficacy in patients with relapsed/refractory B-cell precursor acute lymphoblastic leukemia. However, several studies showed that blinatumomab has a short plasma half-life due to its low molecular weight, and thus its clinical use is limited. Furthermore, multiple trials have shown that approximately 30% of blinatumomab-relapsed cases are characterized by CD19 negative leukemic cells. Here, we design and characterize two novel antibodies, A-319 and A-2019. Blinatumomab and A-319 are CD3/CD19 bispecific antibodies with different molecular sizes and structures, and A-2019 is a novel CD3/CD19/CD20 trispecific antibody with an additional anti-CD20 function. Our in vitro, ex vivo, and in vivo experiments demonstrated that A-319 and A-2019 are potent antitumor agents and capable of recruiting CD3 positive T cells, enhancing T-cell function, mediating B-cell depletion, and eventually inhibiting tumor growth in Raji xenograft models. The two molecules are complementary in terms of efficacy and specificity profile. The activity of A-319 demonstrated superior to that of A-2019, whereas A-2019 has an additional capability to target CD20 in cells missing CD19, suggesting its potential function against CD19 weak or negative CD20 positive leukemic cells.

关键词： B-cell acute lymphoblastic leukemia     bispecific antibody     trispecific antibody     CD19     CD20    

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标题 作者 时间 类型 操作

Alteration of heat shock protein 20 expression in preeclamptic patients and its effect in vascular and

Fanfan Li, Mengzhou He, Meitao Yang, Yao Fan, Yun Chen, Xi Xia, Yin Xie, Dongrui Deng

期刊论文

Expression of heat shock protein gp96 in osteosarcoma and its clinical significance

GUO Weichun, CHEN Anmin

期刊论文

Development of a dual temperature control system for isoprene biosynthesis in

期刊论文

Beneficial effect of arginine vasopressin on hemorrhagic shock through improving the vascular reactivity

LI Tao, YANG Guangming, XU Jing, LIU Jiancang, LIU Liangming

期刊论文

Nonlinear dynamic analysis of functionally graded carbon nanotube-reinforced composite plates using MISQ20

期刊论文

Rolling-element bearings in China: From ancient times to the 20th century

Lie SUN,Ang LI

期刊论文

Centrifuge experiment and numerical analysis of an air-backed plate subjected to underwater shock loading

Zhijie HUANG, Xiaodan REN, Zuyu CHEN, Daosheng LING

期刊论文

“20世纪我国重大工程技术成就” 评选结果在京揭晓

null

期刊论文

ORGANIC CARBON STOCKS IN A SILTY TEXTURED SOIL FOLLOWING REINTEGRATION OF A 20 YEARS OLD × SITE INTO

期刊论文

Green building research from design to operation in the past 20 years: A perspective

Borong LIN, Yi WANG, Jiaping LIU, Juan YU

期刊论文

大秦线开行20 kt级重载组合列车系统集成与创新

耿志修

期刊论文

新型20 m桥梁检测车结构的有限元力学分析

沈意平,何福泉,王送来,张田

期刊论文

Influence evaluation of loading conditions during pressurized thermal shock transients based on thermal-hydraulics

Jinya KATSUYAMA, Shumpei UNO, Tadashi WATANABE, Yinsheng LI

期刊论文

Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction

Hong LI,Ziding ZHANG

期刊论文

Preclinical characterization and comparison between CD3/CD19 bispecific and novel CD3/CD19/CD20 trispecific

期刊论文